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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35286*  Cite 
Description

Freeze substituted Lilium pollen tube immunolabeled for filamentous actin and imaged using laser scanning confocal microscopy. Shown is a projection of x-z slices revealing the cortical distribution of the dense sub-apical actin fringe. This image group also contains a projection of x-y slices of this specimen.

Technical Details

Germinated lily pollen on agar loops was plunge frozen, freeze substituted, labeled with an anti-actin mouse monoclonal antibody followed by Cy-3 goat anti-mouse and imaged with a Zeiss 510 meta confocal microscope using a 63x 1.4 NA objective lens. The image shown is a projection of x-z slices. See Lovy-Wheeler et al. 2005, Planta 221:95-104.

Biological Sources
NCBI Organism Classification
Lilium formosanum
Cell Type
pollen tube
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
pollen tube growth
Attribution
Names
Alenka Lovy-Wheeler
Kathleen L Wilsen
Tobias I Baskin
Peter K Hepler
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35286
Archival Resource Key (ARK)
ark:/b7295/w9cil35286
Grouping This image is part of a group.
Imaging
Image Type
photomultiplier tube (PMT)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
Cy3
Processing History
projection of confocal slices
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
cryofixed tissue
FBbi:00000621
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 532px ——
Y 216px ——