Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35283*  Cite 
Description

The f-actin staining in the lamella of a cultured fibroblast shows common features of adherent cells on a flat substrate. The dominant f-actin organization is in the form of stress fibers, linear bundles of actin filaments ending at cell adhesions to the substrate. In the upper left, actin can be seen modeling the highly dynamic leading edge. There are three patches of ruffles behind the leading edge.

Technical Details

Prepared slide purchased from Molecular Probes imaged with a 60X N.A. 1.4 phase 3 lens on an Olympus IX 70 microscope with a 100W Hg arc lamp for illumination, narrow pass filter blocks for widefield fluorescence microscopy, and a 1.5X relay lens in front of a Cooke Sensicam HQ air cooled CCD camera. Imaged at the Analytical Imaging Facility of the Albert Einstein College of Medicine.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
NIH/3T3
Cellular Component
stress fiber
lamellipodium
ruffle
Biological Context
Biological Process
interphase
Attribution
Names
Michael Cammer
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35283
Archival Resource Key (ARK)
ark:/b7295/w9cil35283
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Fluorescein (FITC)
phalloidin
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.067µm
Y 1024px 0.067µm