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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:35203*  Cite 
Description

This video shows the dynamics of the apical cell surface of individual cells of the enveloping layer in a killifish embryo. The fluorescent actin is seen to polymerize and depolymerize in cyclic waves that extend and retract these surface protrusions. Two neighboring cells from a continuous epithelium are expressing the fluorescent GFP-actin. The expression of this inserted gene is mosaic, with some cells expressing, and others not.

Technical Details

Visualizations taken from intact embryos, injected with GFP-actin plasmid at 1-cell stage. Filmed during gastrulation.

Biological Sources
NCBI Organism Classification
Fundulus heteroclitus
Cell Type
epithelial cell
Cellular Component
actin cytoskeleton
Biological Context
Biological Process
cytoskeleton organization
Attribution
Names
Rachel Fink
Pat Wadsworth
Link
Rachel Fink videos
OTHER
Zebrafish -- The Living Laboratory
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL35203
Archival Resource Key (ARK)
ark:/b7295/w9cil35203
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 324px ——
Y 240px ——