Electron micrograph of keratocyte or fibroblast lamellipodial actin network after unprotected extraction. All examples demonstrate frequent branching of actin filaments. Image corresponds to a single panel from Figure 2d in J Cell Biol. 1999 May 31;145(5):1009-26. All of the panels from Figure 2d can be found as CIL 35060-35068.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).