Licensing
Description
Drosophila melanogaster thoracic indirect flight muscle from a four week-old wild-type animal. A longitudinal section of myocyte is displayed. Sections of parallel myofibrils are visible between densely packed mitochondria.
Technical Details
Tissue was dissected using a freeze-fracture technique, fixed in 2.5% glutaraldehyde in 0.1 M sodium cacadylate buffer, pH 7.4, post-fixed in 2% osmium tetroxide solution, stained in 1% uranyl acetate, and embeddeded in EMbed 812 resin. Sections were post-stained with 2% uranyl acetate and stained with Reynolds's Lead Citrate. Images were obtained using transmission electron microscopy techniques, employing a Phillips CM-100 transmission electron microscope. Images were taken on Kodak EM 4489 film, scanned with a Microtek ScanMaker i900, and post-processed using Adobe Photoshop CS4. This image was taken at a magnification of 11500x.
Biological Sources
- NCBI Organism Classification
- Drosophila melanogaster
- (Common fruit fly)
- Cell Type
- flight muscle cell
- Cellular Component
- myofibril
- mitochondrion
- mitochondrial crista
Biological Context
- Biological Process
- flight
Attribution
- Names
- Catherine Braine
- Marian Rice
Grouping
This image is part of a group.Imaging
- Image Type
- film
- Imaging Mode
- transmission electron microscopy (TEM)
- Parameters Imaged
- electron density
- Source of Contrast
- differences in adsorption or binding of stain
- Visualization Methods
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- digitized film
- Data Qualifiers
- processed data
- suitable for spatial measurements
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- tissue in epoxy resin embedment
- Relation To Intact Cell
- sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1766px | 42.33μm |
| Y | 1832px | 42.33μm |