Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:34537^* Cite

Description

High resolution image of the nucleoli of the macronucleus. Nucleoli have a core of DNA that contains the genes that are transcribed into ribosomal RNA. Assembly of the ribosomal subunits from this processed RNA and from proteins transported into the nucleus through the pores occurs in the nucleoli. A concentric stack of rough ER encircles a mitochondrion. TEM taken on 8/16/67 by R. Allen with Philips 200 operating at 60kV. Neg. 7,200X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis.

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources