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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:31942*  Cite 

Time-lapse confocal experiment showing deltorphin II (100 nM; a selective agonist)-induced receptor internalization in primary caudate putamen neurons isolated from Oprd1-EGFP/EGFP knockin mouse. All agonists triggered the clustering of delta opioid receptor-EGFP (DOR-EGFP; green)(bright spots) along the plasma membrane both in cell bodies and processes. DOR-EGFP clusters then progressively internalized, producing a typical vesicular punctate pattern. After 20 min, the fluorescent spots finally converged into bigger vesicles.

Technical Details

Representative experiment is shown. Cells were seeded in glass-bottom, 32-mm diameter plastic dishes (MatTek) coated with polyL-lysine (Sigma). Fully matured primary neurons (10–14 days in vitro) were used, and receptor internalization studies were performed in the presence of various drugs. Samples were observed under a Leica confocal microscope (SP2 AOBS MP) with objective 63X at 37°C. Images were automatically recorded during 20 min, with increasing time intervals to avoid bleaching effects because of repetitive scanning. Specifically, 20 frames every 10 s followed by 10 frames every 30 s, and then 12 frames every minute were recorded. Reconstituted videos (TIMT; in-house software) contain 42 images and last 2 s.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
primary neuron
caudate putamen neuron
Cellular Component
integral to plasma membrane
endocytic vesicle
Grégory Scherrer
Petra Tryoen-Tóth
Proc Natl Acad Sci. 2006. 103: 9691-9696
Dominique Filliol
Audrey Matifas
Delphine Laustriat
Yu Q. Cao
Allan I. Basbaum
Andrée Dierich
Jean-Luc Vonesh
Claire Gavériaux-Ruff
Brigitte L. Kieffer
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
time lapse microscopy
single-spot confocal microscopy
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——