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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:31940*  Cite 
Description

Time-lapse experiments showing deltorphin II (100 nM; a selective agonist)-induced internalization of delta-opioid receptor–enhanced GFP (DOR-EGFP; green) receptors expressed in HEK 293 cells. The C-terminal DOR-EGFP fusion shows unchanged binding, signaling, internalization and down-regulation properties when expressed in HEK-293 cells.

Technical Details

Representative experiment is shown. Samples were observed under a Leica confocal microscope (SP2 AOBS MP) with objective 63X at 37°C. Images were recorded automatically every 30 s during 60 min.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast
Cell Line
HEK 293
Cellular Component
integral to plasma membrane
endocytic vesicle
Attribution
Names
Grégory Scherrer
Petra Tryoen-Tóth
Published
Proc Natl Acad Sci. 2006. 103: 9691-9696
Pubmed
16766653
OTHER
Dominique Filliol
Audrey Matifas
Delphine Laustriat
Yu Q. Cao
Allan I. Basbaum
Andrée Dierich
Jean-Luc Vonesh
Claire Gavériaux-Ruff
Brigitte L. Kieffer
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL31940
Archival Resource Key (ARK)
ark:/b7295/w9cil31940
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
time lapse microscopy
single-spot confocal microscopy
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 640px ——
Y 512px ——
Time 30 seconds 120