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A confocal 4D-stack from Cycle 11 in a transgenic Drosophila embryo expressing gfp-tubulin (shown in green) and histone-rfp (shown in red). This image is original data contributing to Fig. S1. "Spindle dynamics in Drosophila embryos and in vitro gliding assays with Ncd and KLP61F" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

Technical Details

Embryos expressing gfp-tubulin and histone-rfp were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confcal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
early embryonic cell
Cellular Component
spindle microtubule
kinesin complex
nuclear lamina


Gul Civelekoglu-Scholey
Li Tao
Ingrid Brust-Mascher
Roy Wollman
Jonathan M. Scholey
J Cell Biol. 2010 Jan 11;188(1):49-68.


This image is part of a group.
Data Qualifiers
raw, unprocessed data

Sample Preparation

living tissue
Relation To Intact Cell
whole mounted tissue


Spatial Axis Image Size Pixel Size
X 672px ——
Y 512px ——
Z 4 0.5μm
Time -- sec 90
*CIL – Cell Image Library accession number. Please use this to reference an image.