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Description

A confocal 4D-stack from a transgenic Drosophila embryo during prometaphase expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) and the lamin-B tail dominant negative subfragment. The microinjection of a mixture of the lamin-B tail dominant negative subfragment disrupts the nuclear lamina and causes defects in mitosis. This image is original data contributing to Fig. 6 "Functional perturbation of the lamin-B envelope interferes with spindle length changes and the completion of mitosis" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

Technical Details

Embryos expressing lamin-B-GFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. For injections, embryos were dehydrated for 3-6 min, covered with halocarbon oil and injected (as described in Brust-Mascher & Scholey, 2009). For double injections, embryos were allowed to recover for 5塜 min between injections. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
early embryonic cell
Cellular Component
spindle microtubule
kinesin complex
nuclear lamina

Attribution

Names
Gul Civelekoglu-Scholey
Li Tao
Ingrid Brust-Mascher
Roy Wollman
Jonathan M. Scholey
Published
J Cell Biol. 2010 Jan 11;188(1):49-68.
Link
jcb-dataviewer
Pubmed
20065089

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 672px ——
Y 512px ——
Z 5 ——
Time -- sec 72
*CIL – Cell Image Library accession number. Please use this to reference an image.