Licensing

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Description

Severing of budded yeast. Amoebae (D. discoideum) were plated with yeast (S. cerevisiae) in a glass bottom chamber and viewd by confocal microscopy. The amoebae were expressing mRFP-LimEdelta (red) to label actin filaments and PHcrac-GFP (green) to label the membrane of the nascent phagocytic cup, which is rich in phosphatidylinositol (3,4,5) trisphosphate. The phagocytic cup partially engulfs both lobes of a budded yeast; the cup initially extends beyond the mother-bud neck. The cup then retracts back to the neck, and actin filaments strongly accumulate there, applying force. This situation persists for six minutes, ending with severing of the yeast, sealing of the cup, and ingestion of one lobe of the severed yeast. The time series was collected at 3.94-second intervals using a Zeiss LSM 510 laser scanning confocal microscope equipped with a 63x 1.4 NA DIC objective lens. Pixel size 0.12 x 0.12 microns.

Biological Sources

NCBI Organism Classification
Dictyostelium discoideum
Saccharomyces cerevisiae
(Baker's Yeast, Brewer's Yeast)
Cell Type
amoeboid cell
budded yeast
Cellular Component
actin filament
phagocytic cup

Biological Context

Biological Process
severing of budded yeast

Attribution

Names
Margaret Clarke
Ulrike Engel
Jennifer Giorgione
Annette Müller-Taubenberger
Jana Prassler
Douwe Veltman
Günther Gerisch
Published
BMC Biol. 2010 Dec 29;8:154
Pubmed
21190565

Grouping

This image is part of a group.
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 276px 0.12μm
Y 220px 0.12μm
Time 3.94 sec
*CIL – Cell Image Library accession number. Please use this to reference an image.