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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:30565*  Cite 
Description

Severing of budded yeast. Amoebae (D. discoideum) were plated with yeast (S. cerevisiae) in a glass bottom chamber and viewd by confocal microscopy. The amoebae were expressing mRFP-LimEdelta (red) to label actin filaments and PHcrac-GFP (green) to label the membrane of the nascent phagocytic cup, which is rich in phosphatidylinositol (3,4,5) trisphosphate. The phagocytic cup partially engulfs both lobes of a budded yeast; the cup initially extends beyond the mother-bud neck. The cup then retracts back to the neck, and actin filaments strongly accumulate there, applying force. This situation persists for six minutes, ending with severing of the yeast, sealing of the cup, and ingestion of one lobe of the severed yeast. The time series was collected at 3.94-second intervals using a Zeiss LSM 510 laser scanning confocal microscope equipped with a 63x 1.4 NA DIC objective lens. Pixel size 0.12 x 0.12 microns.

Biological Sources
NCBI Organism Classification
Dictyostelium discoideum
Saccharomyces cerevisiae
Cell Type
amoeboid cell
budded yeast
Cellular Component
actin filament
phagocytic cup
Biological Context
Biological Process
severing of budded yeast
Attribution
Names
Margaret Clarke
Ulrike Engel
Jennifer Giorgione
Annette Müller-Taubenberger
Jana Prassler
Douwe Veltman
Günther Gerisch
Published
BMC Biol. 2010 Dec 29;8:154
Pubmed
21190565
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL30565
Archival Resource Key (ARK)
ark:/b7295/w9cil30565
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
bright-field microscopy
Parameters Imaged
fluorescence emission
absorption of illumination
Source of Contrast
distribution of a specific protein
differences in intrinsic optical density
Visualization Methods
EGFP
mRFP1
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 276px 0.12µm
Y 220px 0.12µm
Time 3.94 seconds


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