Licensing

Attribution_by_nc
Attribution Non-Commercial:This image is licensed under a Creative Commons Attribution, Non-Commercial License. View License Deed | View Legal Code
Comments: Powered by Pnyxe

Description

Dynamics of spindle microtubules and chromosomes during cycle 11 in a transgenic Drosophila embryo expressing GFP-tubulin (green) and histone-RFP (red). Only a single confocal plane containing the centrosomes was acquired during imaging. Images were acquired with exposure times of 1.8 s for GFP and 0.6 s for RFP, and total time shown is 432 s. This time series is the original data from Video 1 "Dynamics of spindle MTs and chromosomes during cycle 11 in a transgenic Drosophila embryo expressing GFPtubulin and histone-RFP" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

Technical Details

Embryos expressing GFP-tubulin and histone-RFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
early embryonic cell
Cellular Component
spindle microtubule
kinesin complex
nuclear chromosome

Attribution

Names
Gul Civelekoglu-Scholey
Li Tao
Ingrid Brust-Mascher
Roy Wollman
Jonathan M. Scholey
Published
J Cell Biol. 2010 Jan 11;188(1):49-68.
Link
jcb-dataviewer
Pubmed
20065089

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Green fluorescent proteins from Aequorea
mRFP1
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 672px ——
Y 512px ——
Time -- sec 168
*CIL – Cell Image Library accession number. Please use this to reference an image.