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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:30563*  Cite 
Description

A confocal 4D-stack from a transgenic Drosophila embryo expressing lamin-GFP (green) and injected with rhodamine-conjugated tubulin (red) during prometaphase shows normal elongation of the spindle during the transition during prometaphase II. This image is original data contributing to Fig. 6 "Functional perturbation of the lamin-B envelope interferes with spindle length changes and the completion of mitosis" from Civelekoglu-Scholey et al.(2010) Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope, J. Cell Biol. 188(1):49-68.

Technical Details

Embryos expressing lamin-B-GFP were collected at 25°C for 1 h, matured for 40 min, dechorionated, placed on heptane glue and covered with halocarbon oil. For injections, embryos were dehydrated for 3-6 min, covered with halocarbon oil and injected (as described in Brust-Mascher & Scholey, 2009). Images from this image group were acquired with an inverted IX-70 Olympus with Ultra-View spinning disk confocal head (PerkinElmer) and acquired with an oil immersion objective (UPlan-Apochromat 100x N.A. 1.35, or Plan-Apochromat 60x NA 1.4). Time series (at intervals of 3 to 10 sec) z-stacks of planes at 0.5 µm were acquired with an Orca II CCD camera (Hamamatsu Photonics). For further information see J. Cell Biol. 188(1):49-68.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
early embryonic cell
Cellular Component
spindle microtubule
kinesin complex
nuclear lamina
Attribution
Names
Gul Civelekoglu-Scholey
Li Tao
Ingrid Brust-Mascher
Roy Wollman
Jonathan M. Scholey
Published
J Cell Biol. 2010 Jan 11;188(1):49-68.
Pubmed
PMID:20065089
Link
jcb-dataviewer
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL30563
Archival Resource Key (ARK)
ark:/b7295/w9cil30563
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Green fluorescent proteins from Aequorea
Rhodamine
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 672px ——
Y 512px ——
Z 9px 0.5µm