Image shows a z-series immuno-FISH of a mouse ES cell carrying a multi-copy alpha-globin BAC insertion: HP1 gamma immunostaining (blue) is shown relative to BAC FISH signal (red) versus combined lac operator, vector backbone FISH signal (green).
Formaldehyde fixed cells were imaged using an IMT-2 Olympus fluorescence microscope with 60x 1.4 NA objective lens. Z-series were recorded, and the slices processed by iterative constrained deconvolution. Pixel dimensions are 0.067 microns (xy) and z spacing is 0.2 microns. See also: P Sinclair et al. 2010 Dynamic plasticity of large scale chromatin structure revealed by self-assembly of engineered chromosome regions. J Cell Biol 109:761-776.
GroupingThis image is part of a group.
- Image Type
- charge coupled device (CCD)
- Imaging Mode
- fluorescence microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- distribution of a specific protein
- differences in adsorption or binding of stain
- Visualization Methods
- 4',6-diamidino-2-phenylindole (DAPI)
- Processing History
- constrained iterative deconvolution
- Data Qualifiers
- processed data
- suitable for spatial measurements
|Spatial Axis||Image Size||Pixel Size|