Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:30546^* Cite

Description

Dictyostelium discoideum (ameoba) phagocytosing budded yeast (S. cerevisiae). The amoeba is expressing mRFP-LimEdelta (labels actin filaments, red) and PHcrac-GFP (labels membranes containing phosphatidylinositol (3,4,5)-trisphosphate and phosphatidylinositol (3,4)-bisphosphate, green) Images were collected with a Zeiss LSM 510 laser scanning confocal microscope equipped with a 63x 1.4 NA DIC objective. The time series was acquired in a single focal plane at 3.94-second intervals. Pixel size is 0.14 x 0.14 microns.

Biological Sources

NCBI Organism Classification: Dictyostelium discoideum; Saccharomyces cerevisiae
Cell Type: amoeboid cell; budded yeast
Cellular Component: actin filament

Biological Context

Biological Process: phagocytosis

Attribution

Names: Margaret Clarke; Ulrike Engel; Jennifer Giorgione; Annette Müller-Taubenberger; Jana Prassler; Douwe Veltman; Günther Gerisch
Published: BMC Biol. 2010 Dec 29;8:154
Pubmed: 21190565

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL30546
Archival Resource Key (ARK): ark:/b7295/w9cil30546

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: single-spot confocal microscopy; bright-field microscopy
Parameters Imaged: fluorescence emission; absorption of illumination
Source of Contrast: distribution of a specific protein; differences in intrinsic optical density
Visualization Methods: EGFP; mRFP1
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	276px	0.14µm
Y	257px	0.14µm

Time	3.94 seconds