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Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:2868*  Cite 
Description

A high resolution image of a longitudinal section of a somatic cilium showing the axoneme, basal body, and attached fibrous and microtubular elements. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. TEM taken on 5/24/69 by R. Allen with Philips 300 operating at 60kV. Neg. 18,500X. The raw film was scanned with a Nikon Coolscan 9000ED. This high resolution image is suitable for quantitative analysis Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Coleps hirtus
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Cambridge Culture Collection, Cambridge, England, catalog # LB1656/1
Cellular Component
cilium axoneme
axonemal microtubule
axonemal dynein complex
plasma membrane
microtubule basal body
cytoskeleton
microtubule cytoskeleton
actin cytoskeleton
bacteroid-containing symbiosome
Biological Context
Biological Process
ciliary or flagellar motility
microtubule cytoskeleton organization
microfibril organization
detection of symbiont
Molecular Function
structural molecule activity
Attribution
Names
Richard Allen
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL2868
Archival Resource Key (ARK)
ark:/b7295/w9cil2868
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 5582px 0.8nm
Y 3792px 0.8nm


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