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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27224*  Cite 

Collagen fibrils in the basal plate of Fundulus heteroclitus scales. The basal plate is made of thick collagen fibrils about 100 nm in diameter, which are organized into a plywood-like structure. Note the changing orientation of the collagen fibrils at every layer of fibrils. During formation of this plywood-like organization, collagen fibrils are synthesized in a direction that is approximately perpendicular to the preceding ply. These well-defined layers of collagen constitute the stratum compactum of the dermis of Fundulus heteroclitus.

Technical Details

Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.

Biological Sources
NCBI Organism Classification
Fundulus heteroclitus
Cell Type
Cellular Component
fibrillar collagen
stratum contractum
Biological Context
Biological Process
extracellular matrix organization
Wai Yan Lam
Marian Rice
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
unprocessed raw data
Spatial Axis Image Size Pixel Size
X 1689px 2.59nm
Y 1781px 2.59nm