NCBI Organism:unidentified plant; Biological process:reproduction

Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27153^* Cite

Description

Z-stack through an unidentified pollen (mixed pollen grain w.m.; Carolina, commercial sample), with 200 image planes. See accompanying image in this group for a colorized maximum projection.

Technical Details

Pollen was imaged using an iMIC Andromeda Spinning Disk Confocal (TILL Photonics GmbH Objective: Olympus UPlanSApo 60X/NA1.35 CCD Camera: Imago QE (PCO). Excitation, 561nm Laser, with the following filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma, custom-made design); and emission filter, FF01-390/482/563/640 (Semrock).

Biological Sources

NCBI Organism Classification: unidentified plant

Biological Context

Biological Process: reproduction

Attribution

Names: Sebastian Rhode, Till Photonics

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL27153
Archival Resource Key (ARK): ark:/b7295/w9cil27153

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy
Parameters Imaged: fluorescence emission
Data Qualifiers: processed data; suitable for spatial measurements

Sample Preparation

Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	622px	0.1075µm
Y	455px	0.1075µm
Z	200px	0.2µm