Licensing

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Description

HeLa cells stably expressing the BAC-encoded GFP-labeled hPrp4 snRNP and MS2-mRED protein and treated with doxycholine to express the E3 transgene. Shown is the GFP signal. Cells imaged witb a Leica SP5 confocal microscope using a 60x 1.4NA HCX Plan Apo objective lens. From Fig 5C in Huranova et al. 2010 J Cell Biol 191:75-86.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
cervical epithelium
Cell Line
HeLa
Cellular Component
pre-mRNA
snRNP
spliceosomal complex

Biological Context

Biological Process
RNA splicing
RNA metabolic process

Attribution

Names
M. Huranova
I. Ivani
A. Benda
I. Poser
Y. Brody
M. Hof
Y. Shav-Tal
K.M. Neugebauer
D. Stanek
Published
M Huranova et al. 2010 J Cell Biol 191:75-86

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mRED
Processing History
line averaging
Data Qualifiers
processed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.