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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26539*  Cite 
Description

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, Swiss3T3 cells were transfected with a plasmid encoding myc-tagged βPIX (green, stained for myc-βPIX) and costained myosin IIA (red). These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is the original data file from Fig. S1, J. Cell Biol. 2010. Vol. 190(4):663–674

Technical Details

Cells were cultured in DME (Invitrogen) supplemented with 10% fetal bovine serum and 100 U/ml penicillin/streptomycin (Invitrogen) at 37°C in a humidified 5% CO2 incubator. For transfections, cells in 60-mm-diameter dishes or on fibronectin-coated coverslips were incu- bated with a mixture of DNA and LipofectAMINE 2000 (Invitrogen) according to the manufacturer’s instructions. The cDNA for βPIX was subcloned into pCMV-myc (Takara Bio Inc.). After 4 h, cells were replated onto fibronectin-coated coverslips for 12h and stained for myc- βPIX (green) and MIIA (red). Images were captured by Zeiss LSM 710 confocal microscope with Plan-Apochromat 63X objective.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
3T3-Swiss albino
Cellular Component
lamellipodium
myosin II complex
actin filament
Biological Context
Biological Process
regulation of cell migration
Attribution
Names
Chan-Soo Lee
Chang-Ki Choi
Eun-Young Shin
Martin Alexander Schwartz
Eung-Gook Kim
Published
J. Cell Biol. 2010. Vol. 190(4):663–674
Pubmed
PMID:20713598
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL26539
Archival Resource Key (ARK)
ark:/b7295/w9cil26539
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
Alexa Fluor 488
Alexa Fluor 546
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——