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Description

This micrograph shows the pegs on the surface of the decorated tubules associated with the contractile vacuole that are negatively stained by the uranyl acetate dissolved in the methylcellulose solution used to give support to the frozen then thawed section. This section was labeled by a polyclonal antibody to the V-ATPase of Dictyostelium which cross-reacts with the V-ATPase in Paramecium. 108:3163-3170, 1995). TEM taken on 2/28/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Adapted with permission from J. Cell Sci. 108:3163-3170, 1995. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of 𷌴°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13123) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuolar membrane
proton-transporting V-type ATPase complex

Biological Context

Biological Process
contractile vacuole organization

Attribution

Name
Richard Allen (University of Hawaii)
Published
J. Cell Sci. 108:3163-3170, 1995
Link
http://www5.pbrc.hawaii.edu/allen/
Pubmed
7593277

Grouping

This image is part of a group.

Imaging

Image Type
transmission electron microscopy (TEM)
illumination by electrons
Imaging Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data

Sample Preparation

Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 1884px ——
Y 1338px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.