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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26266*  Cite 
Description

This micrograph shows the pegs on the surface of the decorated tubules associated with the contractile vacuole that are negatively stained by the uranyl acetate dissolved in the methylcellulose solution used to give support to the frozen then thawed section. This section was labeled by a polyclonal antibody to the V-ATPase of Dictyostelium which cross-reacts with the V-ATPase in Paramecium. 108:3163-3170, 1995). TEM taken on 2/28/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Adapted with permission from J. Cell Sci. 108:3163-3170, 1995. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are outlined in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13123) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuolar membrane
proton-transporting V-type ATPase complex
Biological Context
Biological Process
contractile vacuole organization
Attribution
Names
Richard Allen (University of Hawaii)
Published
J. Cell Sci. 108:3163-3170, 1995
Pubmed
7593277
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL26266
Archival Resource Key (ARK)
ark:/b7295/w9cil26266
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
intrinsic mass distribution
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
gold
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1884px ——
Y 1338px ——


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