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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26252*  Cite 

Radial glial cell from right tectal lobe of Xenopus laevis tadpole CNS, stage 47. This radial glial cell expresses eGFP. It was transfected ~60 hours earlier with in vivo electroporation of plasmid DNA encoding eGFP. Spinning disk confocal microscope (Ultraview system, Perkin Elmer): Yokagowa CSU-X1 spinning disk confocal head mounted on an Olympus BX61WIF microscope with a 60X 1.1 NA objective (LUMFLN 60XW) and an EMCCD camera (Hamamatsu, c9100-50). A 488 nm laser was used with a 547(55) nm filter. Volocity (Improvision/Perkin-Elmer) was the acquisition software. This glial cell was too large to capture in one stack, therefore 2 separate Z-stacks were acquired that overlapped partially. Each stack was projected (max intensity) using ImageJ. The resulting .tifs were aligned and merged in Photoshop. 1 pixel = 0.344 micron.

Biological Sources
NCBI Organism Classification
Xenopus laevis
Cell Type
radial glial cell
Cellular Component
Biological Context
Biological Process
localization in CNS
Jennifer Bestman
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
in vivo electroporation
Relation To Intact Cell
whole mounted tissue