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Description

Elongation of the lateral trunk ectoderm closely matches that of the ventral body side, but shows very little convergence. This time-lapse movie shows the elongation of the lateral region of Platynereis between 53.5 and 59.5 hours post fertilization at 26C. The movie covers a period of 365 min in 73 frames. This is Movie 2 in supporting information for Proc Natl Acad Sci (2007) 104: 2727-2732.

Technical Details

To label cellular outlines, embryos were incubated for 15 min in 5 μM BODIPY564/570 coupled to propionic acid (Invitrogen, Carlsbad, CA) in natural seawater, then rinsed twice in natural seawater and mounted in a 1:1 mixture of natural seawater and 7.5% MgCl2 to prevent muscular contractions. Time-lapse recordings were performed with a PerkinElmer (Wellesley, MA) Ultraview RS System (spinning disk confocal). Embryos were kept during recordings (12 frames per hour) at a constant temperature of 25C or 26C in natural seawater between slide and coverslip separated by two layers of adhesive tape and silicone paste. The chamber was sealed with mineral oil (Sigma, St. Louis, MO).

Biological Sources

NCBI Organism Classification
Platynereis dumerilii
(Dumeril's Clam Worm)
Cell Type
neurecto-epithelial cell

Biological Context

Biological Process
axis elongation
morphogenesis of an epithelium

Attribution

Names
Patrick R. H. Steinmetz
Fabiola Zelada-Gonzáles
Carola Burgtorf
Joachim Wittbrodt
Detlev Arendt
Published
Proc Natl Acad Sci (2007) 104: 2727-2732.
Link
http://intl.pnas.org/content/104/8/272...
Pubmed
17301244

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
time lapse microscopy
Imaging Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
probe for lipid
BODIPY 564/570
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 744px ——
Y 635px ——
Time 300 sec 73
*CIL – Cell Image Library accession number. Please use this to reference an image.