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Polarized cell rearrangement in the Platynereis neuroectoderm visualized by time-lapse imaging. This time-lapse movie shows convergent extension by mediolateral cell intercalation of the ventral neuroectoderm of Platynereis between 53 and 58 hours post fertilization at 25C. The movie covers a period of 300 min in 61 frames and is Movie 1 in supporting information for Proc Natl Acad Sci (2007) 104: 2727-2732.

Technical Details

To label cellular outlines, embryos were incubated for 15 min in 5 μM BODIPY564/570 coupled to propionic acid (Invitrogen, Carlsbad, CA) in natural seawater, then rinsed twice in natural seawater and mounted in a 1:1 mixture of natural seawater and 7.5% MgCl2 to prevent muscular contractions. Time-lapse recordings were performed with a PerkinElmer (Wellesley, MA) Ultraview RS System (spinning disk confocal). Embryos were kept during recordings (12 frames per hour) at a constant temperature of 25C or 26C in natural seawater between slide and coverslip separated by two layers of adhesive tape and silicone paste. The chamber was sealed with mineral oil (Sigma, St. Louis, MO).

Biological Sources

NCBI Organism Classification
Platynereis dumerilii
(Dumeril's Clam Worm)
Cell Type
neurecto-epithelial cell

Biological Context

Biological Process
convergent extension involved in axis elongation


Patrick R. H. Steinmetz
Fabiola Zelada-Gonzáles
Carola Burgtorf
Joachim Wittbrodt
Detlev Arendt
Proc Natl Acad Sci (2007) 104: 2727-2732.


This image is part of a group.


Image Type
recorded image
time lapse microscopy
Imaging Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
probe for lipid
BODIPY 564/570
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data

Sample Preparation

living tissue
Relation To Intact Cell
whole mounted tissue


Spatial Axis Image Size Pixel Size
X 672px ——
Y 512px ——
Time 300 sec 61
*CIL – Cell Image Library accession number. Please use this to reference an image.