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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25707*  Cite 
Description

Time lapse series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using differential interference contrast optics. This time series is grouped in the Cell Image Library with a corresponding movie.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica HCX PLAN APO 100x NA 1.35 DIC objective lens. images were captured at 3 second intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
retinal pigment epithelium
Cell Line
hTERT-RPE1
Biological Context
Biological Process
mitosis
cell proliferation
Attribution
Names
Toshiyuki Hatano
Greenfield Sluder
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25707
Archival Resource Key (ARK)
ark:/b7295/w9cil25707
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.064µm
Y 1024px 0.064µm
Time 3 seconds 4045