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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25700*  Cite 
Description

A low power view of a median transverse section of a green Trichodina that lives on the surface of a freshwater snail collected in central Illinois. In this view the abundant endosymbiotic zoochlorellae are evident. A large food vacuole is present, and the oral membranelles of the oral apparatus is evident. At the outer periphery is the haplokinety distinguished by a single row of ciliated basal bodies, and adjacent is the outer polykinety, composed of three rows of ciliated basal bodies. TEM taken by G. Antipa in 1968 on a Hitachi HU11A operating at 75kV. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 65nm thickness.

Biological Sources
NCBI Organism Classification
Trichodina
Chlorella
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
symbiont-containing vacuole
zoochlorellae
oral apparatus
food vacuole
cytoplasm
Biological Context
Biological Process
response to symbiont
oral apparatus organization
cytoplasm organization
eukaryotic photosynthetic symbiont
Attribution
Names
Gregory Antipa (San Francisco State University)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25700
Archival Resource Key (ARK)
ark:/b7295/w9cil25700
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Dimensions
Spatial Axis Image Size Pixel Size
X 5451px ——
Y 5374px ——