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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25612*  Cite 
Description

Redirection of circulating blood cells by peristalsis in a Drosophila third-instar larva. Live visualization of blood cells within the open body cavity of a Nrg-GFP/;PxnYFP/ larva. Blood-cell flow is generally posteriorward but peristaltic contractions lead to transient redirection of the cells. Time between frames, 0.44 s. Total duration, 10 s. Movie is Movie S4 of Proc Natl Acad Sci (2008). 105: 10017-10022. Movies from this publication include CIL #s 25607, 25608, 25609, 25610, 25611, 25612, 25613, 25614, 25615, 25616, 25617.

Technical Details

Third-instar [w, Nrg-GFP;Pxn-Gal4, UAS-2xeYFP] larvae were mounted dorsal side up on a glass slide with the anterior and posterior ends of the larvae taped down to varying degrees to prevent locomotion. Larvae were imaged on a Leica MZ16FA fluorescent steromicroscope using a Planapo 1.0× objective, and images were captured on a monochrome Leica DFC350FX digital camera. For real-time recording of larval circulation, frames were captured every ≈400 ms over a 4- to 40-s period. Images were obtained by using the Scope-Pro Advanced Acquisition plug-in, and image analysis was carried out with Image-Pro AMS ver. 5.1 Software (Media Cybernetics).

Biological Context
Biological Process
innate immune response
wound healing
Attribution
Names
Daniel T. Babcock
Amanda R. Brock
Greg S. Fish
Yan Wang
Laurent Perrin
Mark A. Krasnow
Michael J. Galko
Published
Proc Natl Acad Sci (2008). 105: 10017-10022.
Pubmed
18632567
Link
http://intl.pnas.org/content/105/29/10017.ful...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25612
Archival Resource Key (ARK)
ark:/b7295/w9cil25612
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EYFP
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 264px ——
Y 416px ——
Time 0.44 seconds 22