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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25541*
Description

A time-lapse movie showing the dorsal pouch formation and migration of heart-anchoring cells (HANCs) in the Drosophila embryo expressing ubi-DE-cad-GFP (Shg-GFP). Shg-positive HANCs corresponding to the leading edge of the dorsal pouch fold inside from the head epidermis at the time of dorsal pouch invagination. They migrate posteriorly toward the heart and, in stage 16, overlap the anterior aorta. Notice a triangular shape of the migrating HANCs cluster. Movie is SI Movie 5 in Proc Natl Acad Sci USA (2008) 105: 2475-2480. Movies from this publication include CIL# 25536, 25537, 25538, 25539, 25540, 25541.

Technical Details

Embryos expressing Shg-GFP were dechorionated in bleach (2 min), rinsed in water, placed on a coverslip in the appropriate orientation, and then mounted in Voltalef 3M oil. The embryos were visualized using a Zeiss LSM 510 Meta confocal microscope. Images compiled from 1.5-mm-spaced optical sections were collected every 6 min during 2 h. Time-lapse series were assembled and analyzed by using Volocity (Improvision) software.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
epithelial cell
heart-anchoring cells
Cellular Component
adherens junction
filopodium
integral to plasma membrane
zonula adherens
Attribution
Names
Monika Zmojdzian
Jean Philippe Da Ponte
Krzysztof Jagla
Published
Proc Natl Acad Sci USA (2008) 105: 2475-2480.
Pubmed
18250318
Grouping This image is part of a group.
Imaging
Image Type
recorded image
3D reconstruction
Image Mode
single-spot confocal microscopy
time lapse microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
3D reconstruction
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 784px ——
Y 576px ——
Z —— 1500µm
Time 360 seconds 20


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