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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25540*
Description

A 3D reconstruction of the cardiac outflow tract (OFT)(horizontal rotation) from a stage-16 wild-type embryo stained for Ladybird early (Lbe; blue), Tinman (Tin; red), and the DE-Cadherin Shotgun (Shg; white). Heart-anchoring cells (HANCs) (in blue) dorsally overlap the aorta. Staining with Tin reveals its expression in the heart and in the cephalic vascular rudiment cells (cvr). Shg is expressed by the cardioblasts, the HANCs, and the ring gland cells. Note that Shg accumulates between HANCs and Lbe-positive cardioblasts (light pink) suggesting formation of adherent junctions. Movie is SI Movie 4 in Proc Natl Acad Sci USA (2008) 105: 2475-2480. Movies from this publication include CIL# 25536, 25537, 25538, 25539, 25540, 25541.

Technical Details

Stage 16 embryos were stained with primary antibodies: mouse anti-Lbe, rabbit anti-Tim, rat anti-DE-cadherin. Species-specific secondary antibodies were conjugated to Alexa Fluor 488, Cy3 or Cy5. The triple stained embryos were visualized using a Zeiss LSM 510 Meta confocal microscope. Images representing 0.5 mm optical sections were compiled and processed by Volocity software (Improvision).

Imaging
Image Type
recorded image
3D reconstruction
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Alexa Fluor 488
Cy3
Cy5
Processing History
3D reconstruction
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 784px ——
Y 576px ——
Z —— 500µm


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