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Description

Whole mount squash of Drosophila polytene chromosome viewed with high voltage EM showing a series of bands and interbands. This micrograph was recorded with a tilt angle of 56 degrees. The accompanying grouped image, taken at 45 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

Technical Details

Fat cells from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid containing 0.1% Triton-X 100, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated and transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film, are transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Biological Sources

NCBI Organism Classification
Drosophila melanogaster
(Common fruit fly)
Cell Type
fat body cell
Cellular Component
polytene chromosome

Attribution

Name
Hans Ris

Grouping

This image is part of a group.

Imaging

Image Type
film
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data

Sample Preparation

Methods
formaldehyde fixed tissue
acetic acid treatment
Relation To Intact Cell
squashed preparation

Dimensions

Spatial Axis Image Size Pixel Size
X 6773px 1.2nm
*CIL – Cell Image Library accession number. Please use this to reference an image.