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Description

Aggregates of maize mosaic virus (MMV, Rhabdoviridae) in cells from hemolymph of the insect vector Peregrinus maidis (planthopper, Hemiptera, Delphacidae). Antibodies to MMV identify aggregates (green/yellow fluorescence) that accumulate mainly around the nuclei (red) of hemocytes. Methods: Hemolymph smears were fixed in paraformaldehyde, labeled with primary antibodies to MMV, and with secondary antibodies: Alexa Fluor-488 (green), and later stained with the nuclear stain propidium iodide (red). Smears were examined with confocal laser- scanning microscope (Leica TCS SP). Associated references: Ammar, E.-D. and S. A. Hogenhout, S.A. (2008). A neurotropic route for Maize mosaic virus (Rhabdoviridae) in its planthopper vector Peregrinus maidis. Virus Research 131: 77-85. Ammar, E.-D. and Nault, L.R. (1985). Assembly and accumulation sites of Maize mosaic virus in its planthopper vector. Intervirology 24: 33-41.

Biological Sources

NCBI Organism Classification
Maize mosaic virus
Peregrinus maidis
Cell Type
hemocyte (sensu Nematoda and Protostomia)
Cellular Component
nucleus
host cell perinuclear region of cytoplasm

Biological Context

Biological Process
viral transmission by vector

Attribution

Names
El-Desouky Ammar
Saskia A. Hogenhout
L. R. Nault

Grouping

This image is representative of a group of similar images.
Data Qualifiers
raw, unprocessed data

Sample Preparation

Methods
formaldehyde fixed tissue
permeabilized tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 1246px 0.141μm
Y 788px 0.141μm
*CIL – Cell Image Library accession number. Please use this to reference an image.