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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24971*  Cite 
Description

High Voltage TEM image showing the series of bands and interbands characteristic of a polytene chromosome. This image of a whole mount preparation of a fat body cell polytene chromosome from Drosophila melanogaster was taken with a tilt angle of 45 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

Technical Details

Fat body cells from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin-acetic acid containing 0.1% Triton-X 100, and squashed on a glass slide. After freezing in liquid nitrogen, the cover slips removed, and the slide thawed and stained with 1% uranyl acetate, rinsed in 50% ethanol, dehydrated and transferred to amyl acetate, and covered with 2% parlodion in amyl acetate. Selected areas of the parlodion film were transferred to formvar coated EM grids, and the parlodion removed by immersion in amyl acetate (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
fat body cell
Cellular Component
polytene chromosome
Biological Context
Biological Process
chromosome organization
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24971
Archival Resource Key (ARK)
ark:/b7295/w9cil24971
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
Methods
formaldehyde fixed tissue
acetic acid treatment
Relation To Intact Cell
squashed preparation
Dimensions
Spatial Axis Image Size Pixel Size
X 6700px 1nm
X 4800px 1nm


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