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Description
Structural differentiation of actin network in lamellipodium. Electron micrograph of Xenopus fibroblasts after regular extraction in the presence of polyethelene glycol (PEG) and phalloidin. While the actin network in the front zone of these cels remains dense, the actin network at the lamellipodial rear disassembled in the course of unprotected extraction (CIL 24800). Image corresponds to Figure 6c from J Cell Biol. 1999 May 31;145(5):1009-26.
Technical Details
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
Biological Sources
- NCBI Organism Classification
- Xenopus laevis
- (African clawed frog)
- Cell Type
- fibroblast
- Cellular Component
- lamellipodium
- actin cytoskeleton
- filopodium
Biological Context
- Biological Process
- cellular localization
- actin filament organization
- Molecular Function
- structural constituent of cytoskeleton
Attribution
- Names
- Tatyana M. Svitkina
- Gary G. Borisy
- Published
- J Cell Biol. 1999 May 31;145(5):1009-26.
- Pubmed
- 10352018
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- transmission electron microscopy (TEM)
- Parameters Imaged
- optical path length gradient
- Source of Contrast
- differences in deposition of metal shadow
- Visualization Methods
- shadowing and plating
- platinum replica
- Processing History
- unprocessed raw data
Sample Preparation
- Methods
- permeabilized tissue
- glutaraldehyde fixed tissue
- phalloidin
- polyethelene glycol
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 4380px | —— |
| Y | 3360px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.