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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24796*  Cite 
Description

Localization of Arp2/3 complex in a fibroblast lamellioidia. Staining with p21 antibody (green) and TRIT-phalloidin (red) shows Arp2/3 complex highly enriched in lamellipodia. CIL 24796 shows an immuno EM image of Arp2/3 localization in a fibroblast. CIL 24794 and 24795 show corresponding images in Xenopus keratocytes. Image corresponds to Figure 3e-g from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
Cell Type
fibroblast
Cellular Component
lamellipodium
actin cytoskeleton
Biological Context
Biological Process
cellular macromolecule localization
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24796
Archival Resource Key (ARK)
ark:/b7295/w9cil24796
Grouping This image is part of a group.
Sample Preparation
Methods
glutaraldehyde fixed tissue
methanol fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1328px ——
Y 512px ——