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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24788*  Cite 
Description

Multiple branching of actin filaments in lamellipodia of vertebrate fibroblasts. This image shows an overview of the leading edge, and CIL 24789 shows enlargements of local regions of this platinum replica. CIL 24786 shows similar overview and local enlargements for the lamellipodia of a Xenopus keratocyte. Image corresponds to Figure 1h from J Cell Biol. 1999 May 31;145(5):1009-26.

Technical Details

Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).

Biological Sources
Cell Type
fibroblast
Cellular Component
lamellipodium
actin cytoskeleton
Biological Context
Biological Process
branching of actin filaments
actin filament-based process
ruffle organization
Attribution
Names
Tatyana M. Svitkina
Gary G. Borisy
Published
J Cell Biol. 1999 May 31;145(5):1009-26.
Pubmed
10352018
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL24788
Archival Resource Key (ARK)
ark:/b7295/w9cil24788
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
optical path length gradient
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
platinum replica
Processing History
unprocessed raw data
Sample Preparation
Methods
permeabilized tissue
glutaraldehyde fixed tissue
phalloidin
polyethylene glycol
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 3286px ——
Y 3268px ——