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Description

Thin section showing nucleolus of a Human neuroblastoma SK-N-SH cell imaged with a FEI Tecnai 20 energy filtered electron microscope at 200 KV and a 20 eV energy window. Overlay of net phosphorus distribution (yellow) and nitrogen minus phosphorus (blue). PML, PML body; FC, fibrillar component of nucleolus; DFC, dense fibrillar component of nucleolus; GC, granular component of nucleolus.

Technical Details

Net phosphorus distribution computed from images taken with energy losses of 155 eV and 155 eV bracketing the phosphorus 'edge', and corresponding nitrogen distribution computed from images taken at 385 eV and 415 eV. See Fig 7D in Politz et al., 2005 Mol Biol Cell 16:3401-3410.

Biological Sources

NCBI Organism Classification
Homo sapiens
(Humans)
Cell Type
neuroblastoma
Cell Line
SK-N-SH
Cellular Component
nucleolus
chromatin

Attribution

Name
Joan Politz
Published
Politz et al., 2005 Mol Biol Cell 16:3401-3410
Link
Molecular Biology of the Cell
Pubmed
15857956
Others
Ilvin Polena
Ian Trask
David Bazett-Jones
Thoru Pederson

Grouping

This image is part of a group.

Imaging

Image Type
charge coupled device (CCD)
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
inelastic scattering
Visualization Methods
Electron energy loss
Processing History
multipanel montage
false color
computed image
Data Qualifiers
processed data

Dimensions

Spatial Axis Image Size Pixel Size
X 435px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.