Licensing

Pd
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Description

Freeze-fracture replica of the unicellular photosynthetic prokaryote Prochloron showing the distribution and size of particles on cleaved thylakoid membranes. This image group includes many other freeze-fracture images of Prochloron. See also: Giddings TH, Withers NW, Staehelin LA.1980 Supramolecular structure of stacked and unstacked regions of the photosynthetic membranes of Prochloron sp., a prokaryote. Proc Natl Acad Sci U S A. 77:352-3566.

Technical Details

Prochloron was collected at Coconut Island, Oahu, Hawaii. Green colonies of didemnid ascidians, Diplosoma virens, were collected from shallow waters. Green spherical cells, identified as Prochloron, were released from the ascidians by gently pressing on the colonies while flushing the surface with sea water. Cells were centrifuged, then rinsed in filtered sea water fixed with glutaraldehyde, infiltrated with glycerol and vitrified. Samples were fractured and replicated using a Balzers BA 360 freeze-etch device and examined and in a JEOL 100C TEM at 50,000X magnification. Images were recorded on film and digitized.

Biological Sources

NCBI Organism Classification
Prochloron
Cellular Component
thylakoid

Biological Context

Biological Process
photosynthesis

Attribution

Names
Thomas H Giddings
Nancy W Withers
L Andrew Staehelin

Grouping

This image is part of a group.

Imaging

Image Type
film
Imaging Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements

Sample Preparation

Methods
freeze_fracture/freeze_etch
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 3349px 0.4nm
Y 4570px 0.4nm
*CIL – Cell Image Library accession number. Please use this to reference an image.