Association of plectin with myosin II. A gelsolin-treated REF-52 cell immunogold labeled for plectin (10 nm). After actin depletion, intermediate filaments with plectin sidearms bind to clusters of myosin filaments. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10c from J Cell Biol. 1996 Nov;135(4):991-1007.