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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:21714*  Cite 
Description

Quick-freeze deep-etch image of two decorated tubules with helically wound subunits on their cytosolic surfaces. Each step in a helical turn appears to be formed of three dyads lying side by side across the row. Each dyad appears to be independent with the other two dyads at each step. Thus each helical row apparently consists of a long series of three dyads lying end to end rather than two rows of subunits. This accounts for the three lines per helix observed in some studies (McKanna, J. Ultrastruct. Res. 54:1-10, 1976. Following the tubules downward in this picture the fracture exposes the inside of one tubule and IMPs exposed here may represent the luminal transmembrane extensions of their much larger heads that appear on the cytosolic surface of the tubule. Additionally, a filled collecting canal lies near the decorated tubules with its P-fracture face exposed that is studded with IMPs. A row of indentations leading to openings to the smooth spongiome and holes in a second row are visible. IMPs appear to have different diameters on this P-face and some are organized into rows. The content of the collecting canal lumen has a uniquely etched pattern distinguishing it from other vacuoles and the cytosol. Adapted with permission from the Journal of Cell Science. TEM taken on 6/22/88 by C. Schroeder with Zeiss 10A operating at 60kV. Neg. 31,500X. Bar = 0.2µm. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:13120) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuole
cytoplasm
Biological Context
Biological Process
contractile vacuole organization
cytoplasm organization
Attribution
Names
Richard Allen (University of Hawaii)
C. Schroeder
Published
J. Cell Sci. 108:3163-3170, 1995
Pubmed
7593277
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL21714
Archival Resource Key (ARK)
ark:/b7295/w9cil21714
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
elevation
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
freeze_fracture/freeze_etch
shadowing and plating
recorded image
film
Print of negative scanned for Photoshop
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 2250px ——
Y 2694px ——