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Description
Quick-freeze deep-etch rotary shadowed replica of a parasomal sac from which a clathrin-coated preendosomal vesicle has pinched off. The typical clathrin cage structure is preserved. Fractured trichocyst at the lower left. TEM taken on 6/7/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 31,500X. Bar = 0.2µm. Adapted with permission from the J. Cell Sci. 101:449-461, 1992. A print of the negative was scanned and processed in Photoshop. This image is best used for qualitative analysis. A high resolution image (CIL:12633) is available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Paramecium multimicronucleatum
- Cell Type
- cell by organism
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cellular Component
- early endosome
- clathrin coat
- trichocyst
Biological Context
- Biological Process
- clathrin coat assembly
- endosome organization
- endosome localization
- endosome transport
Attribution
- Names
- Richard Allen (University of Hawaii)
- C. Schroeder
- Published
- J. Cell Sci. 101:449-461, 1992
- Link
- http://www5.pbrc.hawaii.edu/allen/
- Pubmed
- 1629255
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- electron density
- Parameters Imaged
- elastic scattering of electrons
- electron density
- elevation
- Source of Contrast
- differences in deposition of metal shadow
- Visualization Methods
- transmission electron microscopy (TEM)
- Processing History
- freeze_fracture/freeze_etch
- shadowing and plating
- recorded image
- film
- Print of negative scanned for Photoshop
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- freeze_fracture/freeze_etch
- Relation To Intact Cell
- shadowing and plating
- freeze_fracture/freeze_etch
- isolated subcellular component
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 2099px | —— |
| Y | 2686px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.