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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:14003*  Cite 
Description

Hela cells were treated with control siRNA and synchronized by DTB for 48 hours. Cells were fixed and stained for kinetochore proteins CENP-O (green, Alexa Fluor 488 conjugated secondary Ab) Cells were simultaneously stained for centromeres (CREST, red, Alexa Fluor 568 conjugated secondary Ab) and Hoechst 33342 (blue). Fluorescence microscopy was performed at RT on a confocal microscope (LSM510 Meta; Carl Zeiss, Inc.) equipped with a 100× Plan-Apochromat objective. A 543 nm HeNe laser (5 mW output; detection LP560 nm) was used for detection of Alexa Fluor 568–labeled antibodies. The 488nm line of an Argon laser (25 mW nominal output; detection BP 505–530 nm) was used for analysis of Alexa Fluor 488–labeled antibodies. Hoechst 33258 images were captured using the 364nm line of an ion laser (Enterprise II ML UV; Coherent, Inc.; 80 mW nominal output; detection BP 385–470 nm). Image Reference: PMID 20212317

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
HeLa
Cellular Component
nuclear chromosome
chromosome, centromeric region
Biological Context
Biological Process
cell cycle checkpoint
Attribution
Names
Debaditya Mukhopadhyay
Alexei Arnaoutov
Mary Dasso
Published
JCB 188:681–692, 2010
Pubmed
20212317
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL14003
Archival Resource Key (ARK)
ark:/b7295/w9cil14003
Grouping This image is part of a group.
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px ——
Y 1024px ——
Z 12px ——