Licensing

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Description

Human colon carcinoma HCT 116 cells were transfected with an irrelevant siRNA and then retransfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and treated with 100 µM resveratrol for 4 h. For fluorescence microscopy determinations, cells cultured on coverslips were fixed in paraformaldehyde (4% wt/vol) for 15 min at RT, washed three times in PBS, and mounted with mounting medium (Vectashield). Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 1A, top middle panel, in Morselli et al. J Cell Biol 192: 615-629

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Type
permanent cell line cell
Cell Line
HCT 116
Cellular Component
autophagic vacuole

Biological Context

Biological Process
autophagy

Attribution

Names
Eugenia Morselli
Guillermo Mariño
Martin v. Bennetzen
Tobias Eisenberg
Evgenia Megalou
Sabrina Schroeder
Sandra Cabrera
Paule Bénit
Pierre Rustin
Alfredo Criollo
Oliver Kepp
Lorenzo Galluzzi
Shensi Shen
Shoaib Ahmad Malik
Maria Chiara Maiuri
Yoshiyuki Horio
Carlos López-Otín
Jens S. Andersen
Nektarios Tavernarakis
Frank Madeo
Guido Kroemer
Published
J Cell Biol 192: 615-629
Link
http://jcb-dataviewer.rupress.org/jcb/...
Pubmed
21339330

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
charge coupled device (CCD)
Imaging Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
brightness and contrast adjusted
Data Qualifiers
processed data

Sample Preparation

Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
cultured cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 2048px ——
Y 2048px ——
*CIL – Cell Image Library accession number. Please use this to reference an image.