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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13896*  Cite 
Description

DIC image of cells constitutively expressing Tem1 localized to both spindle pole bodies (SPBs) and expressing Kin4 from the GAL1-10 promoter growing in medium with galactose. Constitutive loading of Tem1 to SPBs allowed cells to progress through the cell cycle despite Kin4 overexpression. Compare to control image CIL# 13897. Image is Fig 5C bottom right panel in J Cell Biol. (2011) 192: 599-614. Other images in Fig 5 include CIL# 13895, 13894, 13897, 13896.

Technical Details

Cells (MATa tem1::KanMX ura3::eGFP-CNM67–TEM1::URA3 kin4::TRP1-pGAL1-10-KIN4) were grown in rich medium containing 2% galactose/ 2% raffinose. Imaging was performed at 25C using a Leica DM6000 microscope equipped with a 100x/1.40 NA oil immersion objective lens, and a digital CCD camera (DFC350, Leica). Pictures were processed with LAS AF (leica) and ImageJ software.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Line
W303
Cellular Component
spindle pole body
Attribution
Names
Mauricio Valerio-Santiago
Fernando Monje-Casas
Published
J Cell Biol. (2011) 192: 599-614.
Pubmed
21321099
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13896
Archival Resource Key (ARK)
ark:/b7295/w9cil13896
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
differential interference contrast microscopy
Parameters Imaged
optical path length gradient
Source of Contrast
boundaries between regions with different refractive index
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 621px 0.0644µm
Y 621px 0.0644µm