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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13598*  Cite 
Description

Mouse hind limb tibialis anterior (TA) muscles were injected with BaCl2 followed by daily systemic administration of rapamycin (Rap) starting 7 days after injury. The animals were sacrificed at different days, and the injured muscles were dissected and cryosectioned followed by hematoxylin (blue) and eosin (pink to red) staining. This image shows the regeneration of muscle tissue fourteen days after the injury with daily systemic administration of Rap from day 7 to day 13. The growth of muscle fibers, stained by eosin and showed as red patches, was impaired as indicated by the smaller average cross-section area of the fibers. Nuclei were stained by hematoxylin in blue. This image corresponds to Fig 10B from JCB 189: 1157-1169, 2010. See also CIL: 13595, 13596, 13597, 13599, and 13600.

Technical Details

TA muscles were isolated by dissection, frozen in liquid nitrogen–cooled 2-methylbutane, and embedded in TBS tissue freezing medium. Sections of 10-μm thickness were made with a cryostat at -20°C, placed on uncoated slides, and stained with hematoxylin and eosin. The stained slides were examined with a microscope (DMI 4000B), and the images were captured with a Fluotar 20× 0.4 NA dry objective (Leica) using a camera (RETIGA Exi). The images were processed as 24-bit colored images using Photoshop.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
skeletal muscle cell
Cellular Component
nucleus
Biological Context
Biological Process
myoblast fusion involved in skeletal muscle regeneration
Molecular Function
mTOR inhibition
Attribution
Names
Yuting Sun
Yejing Ge
Jenny Dmevich
Yong Zhao
Mark Band
Jie Chen
Published
JCB 189: 1157-1169, 2010
Pubmed
20566686
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13598
Archival Resource Key (ARK)
ark:/b7295/w9cil13598
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
bright-field microscopy
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
compartmentalization of stain or label
Visualization Methods
hematoxylin
eosin
Processing History
Brightness and Contrast Adjusted
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
cryostat-sectioned tissue
Fix in liquid nitrogen–cooled 2-methylbutane
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1392px 0.32µm
Y 1040px 0.32µm