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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13438*  Cite 
Description

FISH detection of ERBB2 (fluorescein, green) and MYC (rhodamine, red) in normal breast tissue. Nuclei counterstained with DAPI (blue). Tissue was fixed in formaldehyde, embedded in paraffin, and 4-5 micron sections cut. Stained sections were viewed using an Olympus IX70 microscope with a 60x 1.4 NA objective lens, and images acquired with a Photometrics CoolSnap CCD with a 1024X1024 pixel resolution, with a pixel size equivalent to 0.07427 microns in X and Y. Z-stacks were acquired to cover the thickness of the tissue section with a step size of 0.5 microns in the Z-direction, and subjected to deconvolution using SoftWoRx 3.5.1 software. A maximum intensity projection of the stack is shown. The complete stack is included in this group of images. A portion of this field of view is shown in Fig 1B of Meaburn et al., 2009, J Cell Biol 187:801-812.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
breast
Cellular Component
nuclear chromatin
Biological Context
Biological Process
carcinogenesis
Attribution
Names
Karen Meaburrn
Prabhakar Gudla
Sameena Khan
Stephen Lockeyy
Tom Misteli
Published
Meanurn et al, 2009, J Cell Biol 187:801-812
Pubmed
19995938
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13438
Archival Resource Key (ARK)
ark:/b7295/w9cil13438
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
Rhodamine
Fluorescein (FITC)
4',6-diamidino-2-phenylindole (DAPI)
Processing History
3D-deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
formaldehyde fixed tissue
tissue in paraffin embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 0.074µm
Y 1024px 0.074µm