Licensing

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Description

Microtubules in sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Vegetal view, 28-cell sand dollar embryo show astral microtubules frequently cross spindle midplane before and during anaphase, approach within 1 µm of the equatorial surface before furrowing, and curve inward as the furrow ingresses. Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14â��17°C, which is tolerated well. Corresponds to Fig 1B and second segment of Video 1 in J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources

NCBI Organism Classification
Dendraster excentricus
(Eccentric sand dollar)
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
astral microtubule
cleavage furrow

Biological Context

Biological Process
mitotic anaphase
cytokinesis after mitosis

Attribution

Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data

Sample Preparation

Methods
living tissue
Relation To Intact Cell
whole mounted tissue

Dimensions

Spatial Axis Image Size Pixel Size
X 768px 0.24μm
Y 768px 0.24μm
Time 6 sec 240
*CIL – Cell Image Library accession number. Please use this to reference an image.