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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13398*  Cite 
Description

Microtubules in sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). Vegetal view, 28-cell sand dollar embryo show astral microtubules frequently cross spindle midplane before and during anaphase, approach within 1 µm of the equatorial surface before furrowing, and curve inward as the furrow ingresses. Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C, which is tolerated well. Corresponds to Fig 1B and second segment of Video 1 in J Cell Biol. 2009 Dec 14;187(6):831-45.

Biological Sources
NCBI Organism Classification
Dendraster excentricus
Cell Line
Embryo
Cellular Component
microtubule
ensconsin
astral microtubule
cleavage furrow
Biological Context
Biological Process
mitotic anaphase
cytokinesis after mitosis
Attribution
Names
George von Dassow
Koen J.C. Verbrugghe
Ann L. Miller
Jenny R. Sider
William M. Bement
Published
J Cell Biol. 2009 Dec 14;187(6):831-45.
Pubmed
20008563
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13398
Archival Resource Key (ARK)
ark:/b7295/w9cil13398
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 768px 0.24µm
Y 768px 0.24µm
Time 6 seconds 240