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Description

Z-series of fluorescence images of a Hela cell at mitotic metaphase stained for DNA (blue), Aurora B (green) and tubulin (red). This image is the control for the Bod-1 depletion experiment illustrated in CIL: 13372. Bod-1 is a protein that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis.

Technical Details

Cells were fixed with 3.7% paraformaldehyde or in methanol for 2 min at ?20°C. Mouse antiâ��α-tubulin DM1A (Sigma-Aldrich), and mouse antiâ��Aurora B antibody AIM-1 (BD Biosciences) were used at 1:500. Fluorescently labeled secondary antibodies were all obtained from Jackson ImmunoResearch Laboratories. Image corresponds to Figure 5A from JCB vol. 179 no. 2 187-197.

Biological Sources

NCBI Organism Classification
Homo sapiens
(human)
Cell Line
HeLa S3
Cellular Component
chromosome, centromeric region
spindle
auroroa B

Biological Context

Biological Process
mitosis

Attribution

Names
Iain M. Porter
Sarah E. McClelland
Guennadi A. Khoudoli
Christopher J. Hunter
Jens S. Andersen
Andrew D. McAinsh
J. Julian Blow
Jason R. Swedlow
Published
JCB vol. 179 no. 2 187-197
Link
Journal of Cell Biology
Pubmed
17938248

Grouping

This image is part of a group.

Imaging

Image Type
recorded image
Imaging Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
constrained iterative deconvolution

Sample Preparation

Methods
chemically fixed tissue
Relation To Intact Cell
dispersed cells in vitro

Dimensions

Spatial Axis Image Size Pixel Size
X 340px 0.0663μm
Y 328px 0.0663μm
Z 35 .2μm
*CIL – Cell Image Library accession number. Please use this to reference an image.