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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13164*  Cite 
Description

The transverse microtubular ribbon, consisting of ~3 microtubules, extends to the cell's left of the basal body and rises to the top of the ridge where it ends. Thus the proximal end of a pair of basal bodies is firmly anchored by this “tripod” of accessory structures, the kd, pc mt and tr mt. Presumably this tripod will resist the torque induced on the basal bodies by the movements of the ciliary beat and hold the proximal ends of the basal body in a relatively rigid position. Septa of the alveolar sac system and septal pores are seen in this micrograph. Internally extruded trichocysts also seen in this micrograph. TEM taken on 1/12/73 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 9,900X. Bar = 0.5µm. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:38691) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
trichocyst
Biological Context
Biological Process
cortical cytoskeleton organization
extruded trichocysts
Attribution
Names
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13164
Archival Resource Key (ARK)
ark:/b7295/w9cil13164
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Dimensions
Spatial Axis Image Size Pixel Size
X 1956px ——
Y 2064px ——