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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13161*  Cite 
Description

Transition from 1D to 2D migration: regulation of cellular phenotype by ECM topography. Formation of multiaxial lamellae and cell spreading during the transition from 1D to 2D migration. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 1D surfaces were made by using a 2 photon confocal microscope to pattern a polyvinyl alchohol (PVA) coated coverslip. Following gluteraldehyde quenching, ECM proteins were absorbed onto the PVA surface. Movie is video 6 from JCB 2009, 184:481-490

Technical Details

Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 2 min and shown at 15 frames per second.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
NIH/3T3
Cellular Component
cell
Attribution
Names
Andrew D. Doyle
Francis W. Wang
Kazue Matsumoto
Kenneth M. Yamada
Published
JCB 2009, 184:481-490
Pubmed
19221195
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13161
Archival Resource Key (ARK)
ark:/b7295/w9cil13161
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
phase contrast microscopy
Parameters Imaged
elastic scattering of photons
Source of Contrast
differences in amount of elastic light scattering
Visualization Methods
visualization of contiguous regions
Processing History
custom-made smoothing, sharpening, and convolution filters using MetaMorph software
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 400px 2.4µm
Y 400px 2.4µm
Time 120 seconds