Multiple NIH-3T3 fibroblasts undergoing 1D fibrillar migration. Cells were plated on polyvinyl alchohol (PVA) coated coverslips that had been patterned by ablation with a 2 photon confocal microscope. Following gluteraldehyde quenching, extracellular matrix proteins were absorbed onto the PVA surface. Movie is video 3 from JCB 2009, 184:481-490
Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 4 min and shown at 15 frames per second.
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